Figure 3.

CB₁ receptor expression in the jejunum. CB₁ receptor expression was examined by immunofluorescence in male CD1 mice fed a standard chow or high-fat diet (HFD) for 2 wk (n = 5/group). Tissues were stained for CB₁ with a rabbit anti-CB₁ primary antibody followed by a goat anti-rabbit Alexa Fluor 555 secondary antibody. The CB₁ receptor is highly expressed on enteric nerves in the mucosa. We observed no differences in the expression pattern or intensity of this labeling between groups. We also noted faint punctate staining in chow-fed mice that was virtually absent in the HFD-fed mice. Scale bar: 10 μm.