Figure 9.

Effects of a CB₁ receptor agonist on the acute regulation of small intestinal permeability in 2-wk high-fat diet (HFD)-fed mice. Measurement of the flux of 4-kDa FITC-dextran over time across the jejunum (A and C) and ileum (B and D) relative to vehicle (1% Tween 80 and 2% DMSO in Krebs solution) of male CD1 mice fed a HFD for 2 wk after acute treatment in Ussing chambers with the CB₁/CB₂ receptor agonist CP55,940 (A and B) or pretreatment with the CB₁ antagonist AM6545 for 5 min followed by application of CP55,940 at t = 0 (C and D). FD4 flux across the jejunum was significantly lower in CP55,940 compared with vehicle at 10 min (A, P = 0.0212, t = 2.953, df = 14). FD4 flux across the ileum was significantly lower in CP55,940 compared with vehicle at 10 min (B, P = 0.0089, t = 3.248, df = 14). There was no difference in FD4 flux across the jejunum or ileum when tissues were pretreated with AM6545 before application of CP55,940. **P < 0.01, *P < 0.05 compared with vehicle in two-way ANOVA followed by Bonferroni correction for multiple comparisons on log₁₀-transformed data. Data are mean ± SE, n = 4–8/group.