Figure 18.

Expression of ZO-1 in the ileum after treatment with CB₁ receptor ligands. Tissues from male CD1 mice were stained for ZO-1 with a rabbit anti-ZO-1 primary antibody followed by a goat anti-rabbit Alexa Fluor 555 secondary antibody (n = 5/group). Representative immunofluorescence images of ZO-1 in the ileum of high-fat diet (HFD)-fed mice after acute application of vehicle (1% Tween 80 and 2% DMSO in Krebs solution), CB₁/CB₂ receptor agonist (CP55,940), or CB₁ receptor antagonist (AM6545) in Ussing chambers. Nuclei were counterstained with DAPI. Scale bar: 10 μm.