Table 2.
Calculation of ED50 and TD50 for the 6 Hz test of MRS-2485.
| A. Dose Protection and Toxicity Relationships in Mice for MRS-2485 | |||||
|---|---|---|---|---|---|
|
| |||||
| TEST | TIME, hrs | Dose, mg/kg | Observed Toxicity (Motor Impairment) | Deaths | Fraction of Mice Protected |
|
| |||||
| 6 Hz 44 mA | 1.0 | 17 | none | 0 | 1/8 |
| 6 Hz 44 mA | 1.0 | 30 | none | 0 | 3/8 |
| 6 Hz 44 mA | 1.0 | 60 | none | 0 | 6/8 |
| 6 Hz 44 mA | 1.0 | 100 | none | 0 | 7/8 |
| 6 Hz 44 mA | 24.0 | 75 | observed | 0 | 0/8 |
| 6 Hz 44 mA | 24.0 | 110 | unable to grasp rod | 0 | 1/8 |
| 6 Hz 44 mA | 24.0 | 130 | unable to grasp rod | 0 | 2/8 |
| 6 Hz 44 mA | 24.0 | 140 | observed | 2 | 3/8 |
| 6 Hz 44 mA | 24.0 | 150 | observed | 7 | 8/8 |
| B, Calculation of ED-50 and TD-50 for MRS-2485 | ||
|---|---|---|
|
| ||
| Metric | Test, 6 Hz 44 mA (Protection, ED50) | Test, 6 Hz 44 mA(Toxicity/Motor Impairment, TD50) |
|
| ||
| Time | 1 Hour | 24 h |
| ED50 or TD50 | 39.44 mg/kg | 135.87 mg/kg |
| Confidence Interval | [23.42–60.71] | [124.76–148.31] |
| Slope | 3.05 ± 0.95 (SE) | 18.08 ± 6.32 (SE) |