Table 3.
Calculation of ED50 for corneal-kindled mouse test of MRS-2485.
| A. Corneal Kindled Mouse (CKM) Test for MRS-2485 | ||||
|---|---|---|---|---|
|
| ||||
| Dose, mg/kg | Time, hours | Protection (N/T) | Average Seizure Score | Observed Toxicity (motor impairment, N/T), 3–4 days |
|
| ||||
| 10 | 1.0 | 0/8 | 5.0 | 0/8 |
| 20 | 1.0 | 2/8 | 4.5 | 0/8 |
| 40 | 1.0 | 5/8 | 3.0 | 0/8 |
| 65 | 1.0 | 6/8 | 2.0 | 0/8 |
| 75 | 1.0 | 8/8 | 0.125 | 0/8 |
| Metric | Test, CKM Protection | Observed Toxicity (motor impairment, N/T) |
|---|---|---|
|
| ||
| B. Measurement of ED50 for Protection of Corneal Kindled Mouse (CKM) by MRS-2485 | ||
| Time | 1 h | 3–4 days |
| ED50 or TD50 | 33.74 mg kg | none |
| Confidence Interval | [22.6–45.46] | none |
| Slope | 3.89 ± 0.959 | none |
Cv.