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. Author manuscript; available in PMC: 2022 Aug 23.
Published in final edited form as: Nature. 2021 Sep 29;598(7880):353–358. doi: 10.1038/s41586-021-03934-0

Extended Data Figure 1. Lifespan changes in response to different feeding and fasting regimens.

Extended Data Figure 1.

Light blue boxes on graphs indicate duration of TRF (aqua), IF (medium blue), or iTRF (sky blue) during lifespan. (a) Schematic of different feeding regimens utilized in Drosophila lifespan screen. (b) 12-hour time-restricted feeding (TRF) from day 10 until death shortened female lifespan (top; ad lib, solid gray, n=292; TRF, dashed gray, n=142) and minimally extended male lifespan (bottom; ad lib, solid, n=241; TRF, dashed, n=314). (c) In contrast, TRF from days 10–40 extended female (top; ad lib, solid gray, n=292; TRF, dashed gray, n=150) and male (bottom; ad lib, solid gray, n=241; TRF, dashed gray, n=406) lifespan. (d) 24-hour intermittent fasting (IF) from day 10-death shortened both female (top; ad lib, solid gray, n=145; IF, dashed gray, n=149) and male (bottom top; ad lib, solid gray, n=241; IF, dashed gray, n=276) lifespan. (e) Intermittent time-restricted feeding (iTRF) from day 10-death did not extend lifespan (ad lib, solid gray, n=142; TRF, dashed gray, n=157). (f) iTRF regimen from days 10–40 extended male lifespan (ad lib, solid gray, n=323; TRF, dashed gray, n=382). (See Methods for trials, statistics, and source data; n=number of individual flies; p-values were obtained by log-rank analysis (b-f).