Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 6;29(8-9):1117–1129. doi: 10.1038/s41417-021-00414-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — A–B RT-PCR results showing the transfection efficiency of lentiviral overexpression (OE-TMEM158) and knockdown (shRNA-TMEM158-1, sh-1; shRNA-TMEM158-2, sh-2) and the expression of TMEM158 in U87MG, U251MG, and TJ905 glioma cell lines. C–D CCK-8 results showing the proliferation of U87MG, U251MG, and TJ905 glioma cells in response to upregulating and downregulating TMEM158 expression. E, G Colony-formation assay results showing the colony-formation efficiency of glioma cells after overexpression of TMEM158. F, H Colony-formation assay results showing the colony formation efficiency of glioma cells after silencing TMEM158 expression. I–L The migration ability in U87MG, U251MG, and TJ905 glioma cells in response to overexpression and knockdown of TMEM158 was detected by Transwell migration assay. K and L Show the statistical histogram of the quantification of migrated cells after upregulation and downregulation of TMEM158, respectively. M–P The representative images of Transwell invasion assay for glioma cell lines after gain- and loss-of-function TMEM158. The number of invaded cells is shown in (O and P). Data in (K, L, O, and P) are shown as mean ± SD. Scale bar = 100 μm. (^nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).