REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
P2C-1F11	Ge et al. (2021)	PDB code 7CDI
4A8	Chi et al. (2020)	PDB code 7C2L
CC40.8	Zhou et al. (2022)	PDB code 7SJS
REGN10933	Hansen et al. (2020)	PDB code 6XDG
REGN10987	Hansen et al. (2020)	PDB code 6XDG
VRC01	Zhou et al. (2013)	PDB code 4LST; RRID: AB_2491019
HRP goat anti-human IgG (H + L), polyclonal	ZSGB-BIO	Cat# ZB-2304
Bacterial and virus strains
E. coli DH5α	Takara	Cat# 9057
SARS-CoV-2/wild-type (WT)	Ju et al. (2022)	N/A
SARS-CoV-2/Beta	Ju et al. (2022)	N/A
SARS-CoV-2/Delta	Ju et al. (2022)	N/A
Chemicals, peptides, and recombinant proteins
FreeStyle 293 expression medium	Gibco	Cat# 12338026
SARS-CoV-2 WT S1	Sino Biological Inc.	Cat# 40591-V08H
SARS-CoV-2 WT RBD	Sino Biological Inc.	Cat# 40592-V08B
SARS-CoV-2 WT NTD	Sino Biological Inc.	Cat# 40591-V49H
SARS-CoV-2 WT S2	Sino Biological Inc.	Cat# 40590-V08B
SARS-CoV-2 WT spike	Sino Biological Inc.	Cat# 40589-V08B1
SARS-CoV-2 WT NP	Sino Biological Inc.	Cat# 40588-V08B
SARS-CoV spike	Sino Biological Inc.	Cat# 40634-V08B
MERS-CoV spike	Sino Biological Inc.	Cat# 40069-V08B
HCoV-HKU1 spike	Sino Biological Inc.	Cat# 40606-V08B
HCoV-OC43 spike	Sino Biological Inc.	Cat# 40607-V08B
HCoV-229E spike	Sino Biological Inc.	Cat# 40605-V08B
HCoV-NL63 spike	Sino Biological Inc.	Cat# 40604-V08B
Penicillin-Streptomycin (10,000 U/mL)	Gibco	Cat# 15140163
Ampicillin	Amresco	Cat# 69-52-3
Polyethylenimines (PEIs) 25K	PolySciences	Cat# 23966
Trypsin	Gibco	Cat# 25200–072
Fetal bovine serum	Gibco	Cat# 10099–141C
Dulbecco’s Modified Eagle Medium	Gibco	Cat# 11965–092
HEPES (1M) Buffer Solution	Gibco	Cat# 15630–080
Opti-MEM Reduced Serum Medium	Gibco	Cat# 51985034
Roswell Park Memorial Institute (RPMI) 1640 Medium	Gibco	Cat# 11875–101
DEAE-Dextran hydrochloride	Sigma Aldrich	Cat# D9885-10G
Critical commercial assays
RNeasy Plus Mini Kit	Qiagen	Cat# 74134
QIAquick Gel Extraction Kit	Qiagen	Cat# 28706
Bright-Lite Luciferase Assay System	Vazyme Biotech	Cat# DD1204-03
Single Cell Full Length mRNA-Amplification Kit	Vazyme Biotech	Cat# N712
Gold Hi EndoFree Plasmid Maxi Kit	CWBIO	Cat# CW2104M
TMB substrate	Sangon Biotech	Cat# E661007-0100
Deposited data
Human antibody repertoire	This paper	National Genomics Data Center: HRA002366
Experimental models: Cell lines
HEK293F	Gibco	Cat# R79007
Human: HEK293T	ATCC	Cat# CRL-3216
HEK293T expressing human ACE2	YEASEN Biotech	Cat# 41107ES03
Jurkat-FcγRIIIa-V158 Effector Cells	Vazyme Biotech	Cat# DD1301
Recombinant DNA
pNL4-3.Luc.R-E−	NIH AIDS Reagent Program	Cat# 3418
Software and algorithms
TrimGalore v0.6.0	Trim Galore	https://github.com/FelixKrueger/TrimGalore/
PANDAseq v2.11	Masella et al. (2012)	https://github.com/neufeld/pandaseq
IgBlast v1.17.1	Ye et al. (2013)	https://ncbi.github.io/igblast/
Needleall	A program from EMBOSS package	http://emboss.sourceforge.net/apps/release/6.6/emboss/apps/needleall.html
MEGA v10.2.6	Kumar et al. (2008)	https://www.megasoftware.net/
PyMOL	PyMOL	http://www.pymol.org
Graphpad Prism 8	GraphPad	https://www.graphpad.com/
R	R	https://www.r-project.org/
Other
IMGT Reference directory sets	Lefranc et al. (2005)	http://www.imgt.org/vquest/refseqh. html#VQUEST