Table 2.
Oligonucleotide primer sequences.
| Name | Sequence | Notes/use |
|---|---|---|
| cycA_F | 5’ AATTGAATTCGCAGTAGTGATTGTGTGCCG 3’ | Cloning of wild-type cycA gene |
| cycA_R | 5’ GGCTGCAGCTGAATGTACTCACCGCCCC 3’ | Cloning of wild-type cycA gene |
| cycA_SDM_F | 5’ GCGACCCGGAtcCCGGGGCCAAG 3’ | SDM of cycA to introduce BamHI site |
| cycA_SDM_R | 5’ CTTCCTGCGCGAGCGCCG 3’ | SDM of cycA to introduce BamHI site |
| cycA_SEQ_F | 5’ GTATCCGGGCAGCATAGTCC 3’ | Sequence analysis of cycA deletion |
| cycA_SEQ_R | 5’ CGTGATCTCCTATCTGCGGG 3’ | Sequence analysis of cycA deletion |
| cytC4_F | 5’ AATTGAATTCCCGCACCAACGAATACAAGG 3’ | Cloning of wild-type cytC4 gene |
| cytC4_R | 5’ GGCTGCAGCTTCACGCTCTCCTTCGTGG | Cloning of wild-type cytC4 gene |
| cytC4_SDM_UP_F | 5’ TCTGGGCGGGatcCTCCTCGCCC 3’ | SDM of cytC4 to introduce BamHI site |
| cytC4_SDM_UP_R | 5’ CCGGCCAGAAAGCCAAGCC 3’ | SDM of cytC4 to introduce BamHI site |
| cytC4_SDM_DN_F | 5’ GGCAAGCGGAtcCACGAGATCATGTCGC 3’ | SDM of cytC4 to introduce BamHI site |
| cytC4_SDM_DN_R | 5’ GGTGCGGAAGGCCTTGAG 3’ | SDM of cytC4 to introduce BamHI site |
| cytC4_SEQ_F | 5’ TCTGGTTCACCGACAATCAGG 3’ | Sequence analysis of cytC4 deletion |
| cytC4_SEQ_R | 5’ TTTATGCAGGGTCTGTCCCC 3’ | Sequence analysis of cytC4 deletion |
| pufC_LHS_F | 5’CTCGAGTGGCTGTATCTGGTGCTGG 3’ | Cloning of pufC upstream region |
| pufC_LHS_R | 5’ GAATTCGGTCGAAATGCGAACCGC 3’ | Cloning of pufC upstream region |
| pufC_RHS_F | 5’ GGATCCCGAAGCCCGCGTCAGC 3’ | Cloning of pufC downstream region |
| pufC_RHS_R | 5’ GAATTCACCGCCGCGAAGTAACG 3’ | Cloning of pufC downstream region |
| pufC_SEQ_F | 5’ GGCGGAAAAAGAAGAAGCCG 3’ | Sequence analysis of pufC deletion |
| pufC_SEQ_R | 5’ CAACTGGTATCTGTGGGCCG 3’ | Sequence analysis of pufC deletion |
Underlined, restriction site incorporated into the primer.
Lower-case, deviation from wild-type sequence.