Fig. 4.

Effect of GSK3 inhibitors on nitrite production in LPS-stimulated SIM-A9 cells. SIM-A9 cell (5 × 10.⁵ cells/mL cell density) was stimulated with 100 ng/mL LPS for 24 h, in the presence of the GSK3 inhibitors and SFN. Nitrite production was measured in the cell culture media using the Griess method. Data is expressed as means ± SEM. Group comparisons were drawn using one-way ANOVA, followed by the Student–Newman–Keuls post hoc test; *P-value < 0.05 (relative to the LPS-stimulated group); $P-value < 0.05 (relative to negative [untreated] controls); #P-value < 0.05 (BRD0705 at any given concentration relative to BRD3731 at the corresponding concentration)