Fig. 2.

STZ increases Aβ plaque burden and Aβ levels in the hippocampus and cortex of mice. A Brain sections (n = 8 per group) were stained with anti-Aβ (82E1) antibody recognizing both Aβ40 and Aβ42 to assess Aβ deposition. Representative images are shown on the left panel. Aβ deposits in the cortex and hippocampus were quantified as the percentage of the immunostained area with respect to the total area examined (right panel). Scale bar: 200 μm. B Soluble and insoluble Aβ40 and Aβ42 levels in the cortex of mice (n = 6 per group) were measured using sandwich ELISA and the levels were normalized to brain tissue weight. C The protein levels in the cortex of mice (n = 6 per group) were determined by Western blotting, quantified by densitometry, normalized to Actin, and expressed as values relative to the vehicle control. All values are presented as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, N.S. no significant difference, as determined by Student’s t test