Fig. 4.

STZ induces neuroinflammation in the brains of mice. A GFAP and Iba1 protein levels in the cortex of mice (n = 6 per group) was determined by Western blotting, quantified by densitometry, normalized to Actin, and expressed as a value relative to the vehicle control. B IL-6 and TNF-α mRNA levels in the cortex of mice (n = 5 per group) were determined by real-time PCR analysis. The mRNA levels of IL-6 and TNF-α were normalized to the corresponding expression of GAPDH and expressed as values relative to the vehicle control. All values are presented as the mean ± SD, *p < 0.05, **p < 0.01, as determined by Student’s t test