Fig. 7.

Insulin depletion or IL-6 increases the Sirt2 protein level. A Neuro2a-P301L and B Neuro2a cells were treated with or without insulin (10 nM) in FBS-free medium for 24 h before lysis. C Neuro2a-P301L and D Neuro2a cells were treated with 50 ng/mL IL-6 or vehicle control in FBS-free medium for 30 min before lysis. Protein levels were determined by Western blotting and quantified by densitometry. Quantification of Sirt2 protein levels normalized to Actin. Quantification of phosphorylated (p-) protein levels normalized to total (t-) protein levels and expressed as values relative to the control. All values are presented as the mean ± SEM, *p < 0.05, as determined by Student’s t test; n = 3 per group