Fig. 8.

Sirt2 regulates the phosphorylation levels of tau and ERK in Neuro2a-P301L cells. A Neuro2a-P301L cells were transfected with mock control or Sirt2 vector for 48 h before lysis. B Neuro2a-P301L cells were transfected with the control or Sirt2 siRNA for 72 h before lysis. Protein levels were determined by Western blotting and quantified by densitometry. Quantification of PP1 and PP2A protein levels normalized to Actin. Quantification of phosphorylated (p-) protein levels normalized to total (t-) protein levels and expressed as values relative to the control. All values are presented as the mean ± SEM, *p < 0.05, N.S. no significant difference, as determined by Student’s t test; n = 3 per group