Fig. 9.

ERK inhibitor suppresses Sirt2-mediated tau phosphorylation in Neuro2a-P301L cells. Neuro2a-P301L cells were transfected with mock control or Sirt2 vector for 24 h, treated with 20 µM ERK inhibitor (PD98059) for 24 h, and then lysed. Protein levels were determined by Western blotting and quantified by densitometry. Quantification of phosphorylated (p-) protein levels normalized to total (t-) protein levels and expressed as values relative to the control. All values are presented as the mean ± SEM, *p < 0.05, **p < 0.01, ^#p < 0.05, ^###p < 0.001, N.S. no significant difference, as determined by one-way ANOVA; n = 3 per group