Fig. 3.

DNA-free CRISPR/Cas9 mediated genome editing in tomato multiplexing of SP and SP5G genes. a Structure of SP and SP5G genes. Exons are indicated in black boxes. Primers used for genotyping and sequencing are noted with black arrows. For each gene, sgRNAs (red arrows) were designed, all targeting exon 1. Only one SNP (blue arrow) was found within the amplification region of the SP gene. b Genotyping of first-generation events (M₀) by Sanger Sequencing. The DNA sequence of each allele was aligned to wild type (WT) allele and deletions are shown with hyphens and insertions marked with blue color. Protospacer Adjacent Motif (PAM) is shown in bold