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. 2022 Jul 8;59(9):5856–5873. doi: 10.1007/s12035-022-02945-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — DHCR24 low expression induced tau hyperphosphorylation at specific sites in C8D1A astrocytes. a, b Expression of DHCR24 protein was measured by Western blot of cell lysates from DHCR24 low expression (LE) and overexpression (OE) group, compared with blank control (Blank, BL) and vector groups (Negative control, NC1 or NC2). c Expression of DHCR24 mRNA was verified by quantitative PCR. The values are shown as the means ± SEM, n = 3 per group. d Representative images of Western blot analysis of phosphorylated tau. Phosphorylation of tau at Thr181, Ser199, Thr231, Ser262, and Ser396 sites was measured among all groups. e, f, g, h, i One-way ANOVA analysis of tau phosphorylation level at Thr181, Ser199, Thr231, Ser262 and Ser396 after DHCR24 knockdown or knock-in. The values are shown as the means ± SEM, n = 4 per group, One-way ANOVA, *P < 0.05; **P < 0.01; compared with blank control (blank, BL) and vector groups (negative control, NC1 or NC2)