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. 2022 Jul 8;59(9):5856–5873. doi: 10.1007/s12035-022-02945-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — DHCR24 downregulation disrupted caveolae and activated Ras/MEK/ERK signaling pathway in C8D1A astrocytes. a Expression of cavin1 was measured by Western blot of cell lysates from DHCR24 DHCR24 low expression (LE) and overexpression (OE) groups, n = 4 per group. One-way ANOVA analysis of cavin1 expression level was shown in (b). The values are shown as the means ± SEM, n = 4 for each group. c Ras, total MEK (MEK), phosphorylated MEK (p-MEK), total ERK1/2 (ERK), and phosphorylated ERK1/2 (p-ERK) protein levels were measured by Western blot. One-way ANOVA analysis of their expression among all groups is shown in (d, e, f), n = 4 per group. g Representative images of immunofluorescence staining of p-ERK (Red); Magnification: 20 × , Scale bar: 50 µm. The images were captured at random and analysis of p-ERK fluorescent intensity was shown in (h). One-way ANOVA, *P < 0.05; **P < 0.01; compared with blank control (blank, BL) and vector groups (negative control, NC1 or NC2)