Fig. 6.

Inhibition of MEK/ERK signaling reduced tau hyperphosphorylation in DHCR24 knockdown C8D1A astrocytes. a Western blot analysis was applied to assess the protein level of phosphorylated ERK (p-ERK) and phosphorylated tau. DHCR24 knockdown cells were cultured with MEK inhibitor U0126. After 24 h, the phosphorylation level of tau at Thr181, Ser199, Ser262, Thr231, and Ser396 sites were verified. b, c, d, e, f One-way ANOVA analysis of Western blot results of p-ERK and phosphorylated tau. The values are shown as the means ± SEM, n = 4 for each group. *P < 0.05; **P < 0.01; compared with blank control (0 µM)