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Western immunoblot of β-mercaptoethanol extracts from the wild-type strain (lanes 1 and 4), disrupted PIR4::KanMX4 strain (lane 2), wild-type strain transformed with a multicopy plasmid containing PIR4 (lane 3), mnn1 mnn9 strain (lane 5), and mnn1 mnn9 strain containing the PIR4::KanMX4 disruption (lane 6), probed with a polyclonal antibody raised against the β-mercaptoethanol extract from the mnn1 mnn9 strain.
