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. 2022 Aug 9;10:964662. doi: 10.3389/fchem.2022.964662

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Copyright © 2022 Yin, Fan, Zhou and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ZnO-Ber/H regulated reactive oxygen production induced by DCFH-DA. Nrf2/HO-1/NQO1 signal pathway participated in the process of ZnO-Ber/H combating oxidative damage. (A) Confocal fluorescence microscope examinations of intracellular ROS levels of NIH-3T3. (B) Supernatant detections of SOD from NIH-3T3. (C) Evaluation of NO secretion from NIH-3T3. (D) Supernatant detections of LDH from NIH-3T3. (E) Supernatant detections of MDA from NIH-3T3. (F) Western blot detection of expression of Nrf2, HO-1, and NQO1 proteins in NIH-3T3. (G–I) The relative expression analysis of western blot data. Scale bar: 25 μm. Statistical analysis: *p ≤ 0.05, **p ≤ 0.01.