TABLE 2.

Influence of OprF and SigX on doubling times of P. aeruginosa and P. fluorescens grown in different liquid media with constant aeration

Growth conditionsa	Doubling time (min)
	P. aeruginosa				P. fluorescens
	H103 (wild type)	H636 (OprF−)	H814 (SigX−)	H845 (SigX− + OprF)	OE 28.3 (wild type)	FAJ2026 (OprF−)	FAJ2030 (SigX−)
LB + 8 mM salt	30	54	90	78	ND	ND	ND
LB + 200 mM salt	30	30	54	51	27	27	36
LB + 200 mM salt at 22°C	73	77	125	ND	ND	ND	ND
LB + 500 mM salt	36	NDb	60	54	ND	ND	ND
LB + 500 mM salt + succinatec	36	ND	ND	54	ND	ND	ND
MMd + 200 mM salt	42	51	63	54	38	42	53
MM + 500 mM salt	54	81	63	54	54	ND	55
MM + 500 mM KCl	54	ND	60	60	ND	ND	ND
MM + 500 mM sucrose	54	ND	57	54	ND	ND	ND

^aAll P. aeruginosa cultures were grown at 37°C and P. fluorescens cultures were grown at 30°C unless otherwise noted. 

^bND, not done. 

^c7 mM succinate. 

^dMM, minimal medium (BM2).