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. 2022 Aug 9;13:912147. doi: 10.3389/fmicb.2022.912147

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Copyright © 2022 Miura, Kadoya, Takino, Sano and Akiba.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Concentration and genetic diversity of Rotavirus A (RVA) in water samples from DWTP E. The RVA NSP3 gene was quantitatively detected by real-time RT-PCR and plotted together with cases of rotavirus gastroenteritis reported for the week of sample collection in the prefecture upstream (A). Relative abundance (%) was calculated for RVA G genotypes determined by the VP7 gene (B), P genotypes determined by the VP4 gene (C), and I genotypes determined by the VP6 gene amplified in human (D) and animal (E) nested PCR assays. The value above each column of relative abundance represents the number of OTUs with > 500 reads.