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. 2022 Aug 9;13:912147. doi: 10.3389/fmicb.2022.912147

Figure 2.

Figure 2

Phylogenetic trees of Rotavirus A strains detected in surface water samples collected at DWTP E. Trees were built for partial regions of the VP7 (A), VP4 (B), and VP6 genes (C,D) obtained using human and animal nested PCR assays with maximum likelihood and bootstrapped with 1,000 repetitions. The sequence lengths of VP7, VP4, and human and animal VP6 were 242, 230, 239, and 274  bp, respectively. Circles with branch colors represent reference sequences that are closely related to OTU sequences. White and gray circles represent reference sequences for RotaTeq® and closely-related OTU sequences. The information of reference sequences is provided in Supplementary Table S3 in Supplementary material. Triangles at the nodes represent bootstrap rates of >0.8. The name of each sub-cluster consists of the genotype, followed by host or RotaTeq®, 1 (cluster with reference sequences) or 2 (cluster with only OTU sequences), and a or b for more than two sub-clusters. The values represent the minimum/maximum sequence similarity within each sub-cluster.