Table 1.

Oligonucleotide primers specific to VP7, VP4, and VP6 RVA genes.

Name	Outer/inner Fwd./rev.	Sequence (5′ − 3′)	Locationa	Product size
Human VP7
H7F292_N	Outer fwd.	TGYYTRTAYTAYCCWDBHKMRG	292–313	347
H7F340_N	Outer fwd.	TGGAMRRAYWCDYTDTCNCAR	340–360	299
H7R638_N	Outer rev.	GGACAHACTTTHAYNGTRCA	619–638	−
H7F373	Inner fwd.	AARGGDTGGCCRACWRRATC	373–392	239
H7R611	Inner rev.	CCCATHGMWATCCAYTTRTTYGM	589–611
Human VP4
H4F793_N	Outer fwd.	TGGAARGARATGCARTATAAY	793–813
H4R1149_N	Outer rev.	RCABWYYACDKMRTTYAAATY	1,129–1,149	357
H4R1208_N	Outer rev.	CCHCCDBTCATHAYTGGSY	1,190–1,208	416
H4F865	Inner fwd.	GGWYTRGGHTATAARTGGKCHGAA	865–888	229
H4R1093	Inner rev.	ATGCYTKHGAATCRTCCCAR	1,074–1,093
Human VP6
H6F648_N	Outer fwd.	GARCAYRTWGTMCAGCTHMG	648–667	462
H6R1109_N	Outer rev.	YGGRAADACTGGTCCAACTGGTAT	1,086–1,109
H6F745	Inner fwd.	CWGAYGGMGCRACTACATGG	745–764	250
H6R994	Inner rev.	ARYGTVAGTCCWACTGTWGC	975–994
Animal VP6
A6F192_N	Outer fwd.	AGAAAYTGGAMWTTYRAYTTYGG	192–214	457
A6R648_N	Outer rev.	CAAAYTGYTGHRTATTWGCTGG	627–648
A6F303	Inner fwd.	AATGTRTGYATGGATGARATRGYWMGR	303–329	272
A6R574	Inner rev.	GCRTTHADCCACATNGTNCC	555–574

^aLocations of the NCBI reference nucleotide sequence (accession no. NC011503.2 for human VP7, NC011510.2 for human VP4, and NC011509.2 for both human and animal VP6) are represented.