Table 2.
Sensitivity of developed nested PCR assays evaluated using Sample Set 1.a
| Human VP7 | Human VP4 | Human VP6 | Animal VP6 | |||||
|---|---|---|---|---|---|---|---|---|
| Sample month | MNV recovery rate [%] | Conc. [log10 copies/L]b | H7F292_N/H7R638_N | H7F340_N/H7R638_N | H4F793_N/H4R1149_N | H4F793_N/H4R1208_N | H6F648_N/H6R1109_N | A6F192_N/A6R648_N |
| 2019/4 | 71 | 5.5 | − | + | + | + | + | + |
| 5 | 50 | 5.2 | − | + | + | + | + | + |
| 6 | 37 | 5.1 | − | + | + | + | + | + |
| 7 | 32 | 4.9 | − | + | − | + | + | + |
| 8 | 13 | 5.2c | − | − | − | + | + | + |
| 9 | 20 | 4.1 | − | − | − | + | + | + |
| 10 | 68 | 4.8 | − | − | − | + | + | + |
| 11 | 77 | 4.2 | − | − | − | + | + | + |
| 12 | 29 | 4.3 | − | − | − | + | + | + |
| 2020/1 | 31 | 4.3 | − | − | − | + | + | + |
| 2 | 32 | 4.8 | − | − | − | + | + | + |
| 3 | 30 | 4.7 | − | − | − | + | + | + |
a
+/−, positive/negative for target gene.
b
Concentrations determined by quantifying the NSP3 gene.
c
0.5 l was processed due to high turbidity (34 turbidity units of polystyrene latex).