Table 3.
Sensitivity of developed nested PCR assays evaluated using Sample Set 2.a
| Human VP7 | Human VP4 | Human VP6 | Animal VP6 | |||||
|---|---|---|---|---|---|---|---|---|
| Sample month | DWTP ID | MNV recovery rate [%] | Conc. [log10 copies/L]b | H7F340_N/H7R638_N | H4F793_N/H4R1149_N | H4F793_N/H4R1208_N | H6F648_N/H6R1109_N | A6F192_N/A6R648_N |
| 2018/1 | A | 30 | 4.1 | − | + | − | + | + |
| G | 43 | 5.3 | + | + | − | + | + | |
| R | 28 | 5.4 | + | + | − | − | + | |
| U | 36 | 6.3 | + | + | − | + | + | |
| 2018/9 | A | 44 | 4.3 | + | + | − | + | + |
| G | 33 | 5.0 | − | + | − | + | + | |
| R | 44 | 4.7 | − | + | − | + | + | |
| U | 33 | 5.7 | − | + | − | + | + | |
| 2018/10 | O | 47 | 4.7 | + | + | − | − | + |
| 2019/1 | G | 34 | 5.0 | − | + | − | + | + |
| 2019/2 | A | 45 | 4.5 | − | + | − | + | + |
| 2019/10 | C | 20 | 4.6 | + | + | − | + | + |
| G | 29 | 3.5c | − | − | − | − | − | |
| 2020/1 | A | 80 | 4.1 | + | + | − | + | + |
| C | 33 | 4.5 | + | + | + | + | + | |
| D | 32 | 3.7 | − | + | + | + | + | |
| 2020/10 | A | 21 | 3.4 | + | − | + | + | + |
| U | 10 | 4.5 | − | − | − | + | + | |
a
DWTP, drinking water treatment plant; +/−, positive/negative for target gene.
b
Concentrations determined by quantifying the NSP3 gene.
c
0.2 l processed due to high turbidity (219 turbidity units of polystyrene latex).