Table 1.
Reactive Species | Application | Antibody List (Information) |
---|---|---|
mouse | IHC | Against CD163 (ab182422, Abcam, Cambridge, United Kingdom), iNOS (E-AB-70051, Elabscience, Houston, TX), CD206 (ab64693, Abcam), cytokeratin 14 (ab181595, Abcam), Ly6G/6C (ab2557, Abcam), MMP9 (ab228402, Abcam), CD3 (ab16669, Abcam), MOMA2 (ab33451, Abcam), and αSMA (ab32575, Abcam). |
mouse | Flow | APC-conjugated anti-mouse F4/80 (17-4801-82, eBioscience, San Diego, CA) and PE-conjugated anti-mouse CD163 (12-1631-82, eBioscience) and 7-AAD (A1310, Thermo Fisher Scientific, Waltham, MA) |
human | Western blot | p-STAT3 antibody (number 9131S), STAT3 antibody (number 9139S), p-Akt antibody (number 9271), and Akt antibody (number 9272S), Cell Signaling Technology, Danvers, MA). Proteins were detected by enhanced chemiluminescence. |
human | Flow cytometry | PE CF594‒conjugated anti-hCD68 (BD Biosciences, Franklin Lakes, NJ), FITC-conjugated anti-hCD86 (BioLegend, SanDiego, CA), PE/Dazzle 594‒conjugated anti-hCD80 (BioLegend), Pacific Blue-conjugated anti-hCCR7 (BioLegend), PE-conjugated anti-hCD163 (BioLegend), BV605-conjugated anti-hCD206 (740417, BD Bioscience), and BV510-conjugated anti-FVS (564406, BD Biosciences). |
Abbreviations: αSMA, α-smooth muscle actin; 7-AAD, 7-aminoactinomycin D; APC, allophycocyanin; Akt, protein kinase B; IHC, immunohistochemistry; iNOS, inducible nitric oxide synthase; MMP9, matrix metalloproteinase 9; p-Akt, phosphorylated protein kinase B; PE, phycoerythrin; p-STAT3, phosphorylated signal transducer and activator of transcription 3; STAT3, signal transducer and activator of transcription 3.