Reference or Source	Identifier or Catalog Number
Experimental Models
Mice for dissection of embryonic cortical neurons	EMBL Animal House	CD1
Mouse embryonic stem cells	Laboratory of Kyung‐Min Noh	129XC57BL/6J generated from male 129‐B13 agouti mice
Human induced pluripotent stem cells	Michael Snyder lab, Stanford University	male H10‐CESCG‐297
Recombinant DNA
pLV‐TetO‐hNGN2‐eGFP‐Puro	Addgene	Cat# 79823
FUdeltaGW‐rtTA	Addgene	Cat# 19780
pMD2.G	Addgene	Cat# 12259
psPAX2	Addgene	Cat# 12260
pspCas9(BB)‐2A‐GFP	Addgene	Cat# 48138
pspCas9(BB)‐2A‐RFP	Addgene	Cat# 91854
Antibodies
Mouse‐anti‐Map2 mAb	Sigma	Cat# m9942
Chicken anti‐Synaptophysin pAb	Synaptic Systems	Cat# 101 006
Mouse‐anti‐TUBBIII mAb	Abcam	Cat# ab78078
Rabbit anti‐PSD95 pAb	Cell Signaling Technology	Cat# 3450
Camelid sdAB FluoTag‐X4 anti‐GFP labeled with ATTO488	Synaptic Systems	Cat# N0304‐At488‐L
Guinea pig anti‐MAP2 pAb	Synaptic Systems	Cat# 188 004
Guinea pig anti‐Synapsin1/2 pAb	Synaptic Systems	Cat# 106 004
Mouse anti‐Tau monoclonal AB	Novus	Cat# NBP2‐50051
Rabbit‐anti‐Egr1 mAb	Cell Signaling	Cat# 4153
Rabbit‐anti‐c‐Fos mAb	Cell Signaling	Cat# 2250
Goat‐anti‐rabbit IgG conjugated with AlexaFluor 594	Life Technologies	Cat# A11012
Goat‐anti‐mouse IgG conjugated with AlexaFluor 594	Life Technologies	Cat# A11005
Donkey anti‐chicken IgY (IgG) (H+L) conjugated with Alexa Fluor 488	Jackson ImmunoResearch Labs	Cat# 703‐545‐155
Cy3 donkey anti‐rabbit IgG (H+L)	Jackson ImmunoResearch Labs	Cat# 711‐165‐152
Donkey anti‐guinea pig IgG (H+L) conjugated with Alexa Fluor 647	Jackson ImmunoResearch Labs	Cat# 706‐605‐148
Goat anti‐mouse IgG (H+L) conjugated with Alexa Fluor 750	ThermoFisher	Cat# A‐21037
Chemicals, enzymes and other reagents
Accutase	ThermoFisher	Cat# A1110501
Benzonase	Millipore	Cat# 71206‐3
Poly‐D‐Lysine	Sigma	Cat# P0899
Laminin	Sigma	Cat# 11243217001
Neurobasal medium	ThermoFisher	Cat# 21103049
Penicillin/streptomycin	ThermoFisher	Cat# 15140122
GlutaMAX	ThermoFisher	Cat# 35050
B27 supplement	ThermoFisher	Cat# 12587010
Leukaemia Inhibitory Factor (LIF)	EMBL Protein Expression and Purification Core Facility
Retinoic acid	Sigma	Cat# R26250
N‐2 supplement	ThermoFisher	Cat# 17502048
DMEM	ThermoFisher	Cat# 11960085
Essential 8 medium	ThermoFisher	Cat# A1517001
Vitronectin	ThermoFisher	Cat# A14700
Neurobasal A	ThermoFisher	Cat# 10888022
DMEM/F12	Gibco	Cat# 51300‐044
Doxycycline	Sigma	Cat# D9891
Puromycin	Sigma	Cat# P8833
Fibronectin	Sigma	Cat# F0895
Neurobasal medium	ThermoFisher	Cat#21103049
L‐Ascorbic Acid	Sigma	Cat# A5960
DAPT	Tocris	Cat# 2634
CalPhosTM Mammalian Transfection kit	Takara	Cat# 631312
Formaldehyde	ThermoFisher	Cat# 28906
Prolong Gold	ThermoFisher	Cat# P36934
D‐2‐amino‐5‐phosphonopentanoic acid (D‐AP5)	Tocris	Cat# 0106
Tetrodotoxin (TTX)	Tocris	Cat# 1078/1
Brain Derived Neurotrophic Factor (BDNF)	R&D systems	Cat# 248‐BDB
T‐5224	Cayman	Cat# 22904
TurboTM DNase	ThermoFisher	Cat# AM2238
MultiScribeTM Reverse Transcriptase	ThermoFisher	Cat# 4311235
SYBR Green Master Mix	Applied Biosystems	Cat# 4309155
cOmplete protease inhibitor tablets	Roche	Cat# 11 873 580 001
Dynabeads protein G magnetic beads	ThermoFisher	Cat# 10003D
RNase A	ThermoFisher	Cat# EN0531
Proteinase K	ThermoFisher	Cat# EO0491
NEBNext Ultra II kit	New England Biolabs	Cat# E7645L
oligo‐dT capture kit	New England Biolabs	Cat# S1550
RNeasy kit	Qiagen	Cat# 74104
AMPure XP magnetic beads	Beckman Coulter	Cat# A63880
Sera‐Mag SpeevBeads	GE Healthcare	Cat# 45152105050250 and # 65152105050250
Filter plates	MSGVN22	Cat# MSGVN22
TMT10plex	ThermoFisher	Cat# 90110
TMT11	ThermoFisher	Cat# A37724
OASIS HLB plates	Waters	Cat# 186001828BA
Acclaim C18 PepMap 100	ThermoFisher	Cat# 164946
nanoEase M/Z HSS C18 T3	Waters	Cat# 186009249
Other
Real‐Time PCR	Applied Biosystems	ABI7900
NextSeq 500 platform	Illumina	EMBL Heidelberg GeneCore Facility
Nextera DNA Library Prep Kit	Illumina	Cat # FC‐121‐1030
High Sensitivity DNA Bioanalysis Kit	Agilent	Cat# 5067‐4626
Qubit dsDNA HS Assay Kit	ThermoFisher	Cat# Q32851
NEBNext High‐Fidelity 2X PCR Master Mix	New England Biolabs	Cat# M0541S
Subcellular Protein Fractionation for Cultured Cells kit	ThermoFisher	Cat# 78840
Ultimate 3000 HPLC	Dionex
Ultimate 3000 RSLC	ThermoFisher
Q Exactive Plus Mass Spectrometer	ThermoFisher
Nikon Ti‐2 widefield microscope	Nikon