FIGURE 3.

EA treatment reversed WD-TBI-induced elevations in HDAC1 and HDAC3 expression in murine cortex tissue. (A) Timeline for the study procedures including TBI, anesthesia, EA, EA-NAP, and recovery. Mice were sacrificed 48 h after brain injury. (B) Representative Western blot results show levels of HDAC1, HDAC3, and HDAC2 protein expression in murine cortex 48 h after WD-TBI. The brains were removed and the entire cortex was dissected for Western blotting analysis as described in the “Materials and methods.” β-Actin was used as the loading control. (C) Quantitative analysis of HDAC1/3/2 protein expression levels in (B). (D) Representative images of IHC staining show anti-HDAC3 antibody immunoreactivity in the cortical and hippocampal areas of the Control, WD, WD+EA, and WD+EA-NAP groups. High magnification images from the top panels (red box) are shown in the green and blue boxes, respectively. Scale bars of red box panels are 500 μm; of the green and blue box panels are 100 μm. Data are the means ± S.E.M. of at least three independent experiments. *p < 0.05, **p < 0.01 (statistical testing was performed by one-way ANOVA/Newman-Keuls test). The number underneath each bar in (C) refers to the number of mice used in the study group. The original Western blot images are presented in Supplementary Figure 4. The number underneath each bar in (C) refers to the number of mice used in the study group. The F values of one-way ANOVA for (C) are presented in Supplementary Table 2.