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. 2022 Aug 9;16:880267. doi: 10.3389/fncel.2022.880267

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Copyright © 2022 Hung, Chung, Luo, Chu, Chen, MacDonald, Chien, Kotha, Yang, Hwang, Dun, Chuang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EA treatment reduced TNF-α and IL-6 expression in the WD-TBI mouse cortex. (A) Representative Western blot results show levels of TNF-α expression in the cortex from the Control, WD, and WD+EA groups 48 h after TBI. β-Actin was used as the loading control. (B) Quantitative analysis of TNF-α protein expression levels in (A). (C,D) The relative mRNA expression levels of TNF-α and IL-6 in the cortex 48 h after TBI were evaluated by qPCR. Data are the means ± S.E.M. of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (statistical testing was performed by one-way ANOVA/Newman-Keuls test). The number underneath each bar in (B–D) refers to the number of mice used in the study group. The original Western blot images are presented in Supplementary Figure 6. The F values of one-way ANOVA for (B–D) are presented in Supplementary Table 4.