Figure 2.

Exogenous EPO promoted endotoxin-tolerant re-programming.(A, B): In vitro cultured RAW264.7 macrophages (A) or BMDMs from WT C57BL/6 mice (B) were incubated with different dose of rhEPO (0, 10, 20, and 40 IU/ml) in the presence of LPS (100 ng/ml) for 24 h, and then cells were washed with PBS twice followed by a secondary LPS stimulation (10 ng/ml) for 6 h; gene expression was measured by qRT-PCR (n = 3). (C–F): WT C57BL/6 mice were intraperitoneally injected with LPS (1 mg/kg) together with rhEPO (5,000 IU/kg) or PBS for 24 h, and then mice were intraperitoneally given with a secondary LPS injection (10 mg/kg) for 6 h. Control mice were injected with PBS only. (C): Lung specimens stained with H&E (bar = 100 μm, n = 3). (D–F): qRT-PCR assay of gene expression in mice’s lung (D), liver (E), and kidney (F) (n = 3). Data are representative of three independent experiments. Results were expressed as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Statistics: one-way ANOVA (A, B) or two-way ANOVA (D-F) with Tukey’s post-hoc test for multiple comparisons.