Table 1.
FTIR spectroscopy on cellular protein aggregates reveals characteristic spectral features of different β-structures
| Bands (cm−1) | Assignment | Sample | Technique | Reference |
|---|---|---|---|---|
| Tissue-derived aggregates | ||||
| 1620–1618 | Amyloid | p53 (breast and lung carcinoma) | FTIR | (152) |
| ↑1627 | Inter β-sheet (more amyloid) | Mouse advanced age (AD brain tissue) | μFTIR | (153) |
| 1626 | Amyloid | Human AL amyloidosis (heart muscle and heart fat) | ATR-FTIR | (154) |
| 1620/1628/↓1690 | Fibrils (parallel β-sheet) | Aβ plaque core (AD brain tissue) | FTIR | (87) |
| 1630/1692 | Oligomers (Antiparallel β-sheet) | SOD1 mutant (rat model of ALS) | μFTIR | (80) |
| Cell-derived aggregates | ||||
| 1632 | Inter β-sheet | Transthyretin exposed cell | μFTIR | (155) |
| ↓1640 | ↓ Native β-sheet | μFTIR | ||
| ↑1620–1635 | Amyloid | Aβ (AD nerve cell) | μFTIR | (156) |
| 1625 | Inter β-sheet | Ataxin-3 variants (intact cell) | μFTIR | (85) |
| 1642–1640/1630 | Native β-sheet (oligomers) | |||
| Cell-derived IBs | ||||
| 1622 | Amorphous aggregates | Asparaginase (induction at 37–42 °C) | ATR-FTIR | (19) |
| 1628–1629 | Amyloid aggregates | Asparaginase (induction at 20–30 °C) | ATR-FTIR | |
| 1632 | Coiled-coil | ZapB | ATR-FTIR | (71) |
| 1651 | α-helix | ATR-FTIR | ||
| 1620–1630 | Inter β-sheet | Lipase | FTIR | (157) |
| 1650 | α-helix | |||
| 1680 | β-turn | |||
| 1623 | Aggregates | Phospholipase A2 | ATR-FTIR | (158) |
| 1634 | Native β-sheet | |||
| 1650 | α-helix | |||
| 1625 | Amyloid | VP1-GFP in Pichia pastoris | ATR-FTIR | (159) |
| 1680 | Antiparallel β-sheet | |||
| In vitro-derived aggregates | ||||
| 1622 | Inter β-sheet | SOD1 fibers (60 °C + shaking) | FTIR | (160) |
| 1618 | Inter β-sheet | Human Serum Albumin (heated) | ATR-FTIR | (161) |
| 1630/1690 | Amyloid (parallel β-sheet) | Aβ42 fibril | ATR-FTIR | (79) |
| 1630/1690 | Oligomers (Antiparallel β-sheet) | Aβ42 oligomer | ATR-FTIR | |
Details of recent FTIR analyses are compiled ↑ denotes an increase in band signal, ↓ denotes a decrease of band signal.
Abbreviation: μFTIR, FTIR microspectroscopy.