Figure 1.

MBCs in influenza infection

(A) Experimental approach.

(B) Contour plots displaying YFP⁺ and YFP⁻ B cells in Aid-EYFP animals treated as in (A).

(C) Contour plots showing MBCs (CD38⁺GL7⁻) and GC (CD38⁻GL-7⁺) cells gated from the CD19⁺YFP⁺ population in (B).

(D) Contour plots showing MBC binding to NP and HA.

(E) Histograms depicting IgD expression by YFP⁺ MBCs and YFP⁻ B cells.

(F) Histograms showing the labeling of YFP⁺ MBCs and YFP⁻ B cells with anti-CD45 antibody administered i.v. The quantification shows B cells protected from staining.

(G) Absolute numbers of YFP⁺ MBCs in mice treated as in (A) at indicated time points.

(H) Contour plots displaying YFP⁺ B cells in mice infected with 5 PFU of PR8 virus or challenged with 10⁵ PFU of UV-inactivated PR8 virus. Unless indicated, all analyses were performed on day 70 of the infection. In all the panels, bar charts show the quantification of one representative experiment out of three, mean ± SEM. Each dot represents one mouse. t test: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001.

See also Figure S1.