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. 2022 Jul 1;18(2):445–450. doi: 10.4103/1673-5374.346550

Figure 6.

Figure 6

Sox11b regulates the expression of Notch signaling components in injured retinas.

(A, B) qPCR revealed the relative expression levels (normalized to 0 days) in the retina at 4 dpi: Notch ligands (A); Notch receptors and the reporter gene her4.1 (B). (C) BrdU immunofluorescence showed that RO4929097-treated MGPCs exhibited a more rounded morphology. Outlines of MGPC nuclei are indicated by white dotted lines. (D) Quantification of MGPC nuclear length/width ratio in the INL. (E) BrdU lineage tracing indicated that Notch inhibition altered the distribution and increased the number of MGPCs, compared with control retinas at 7 dpi. White asterisks indicate sites of stab injury. (F) Quantification of MGPC cell number at the injury site. (G) Quantification of the proportion of BrdU+ cells in each layer. (H) qPCR showed the relative mRNA expression levels (normalized to 0 days) of sox11a and sox11b in PBS- or RO4929097-treated retinas at 4 dpi. Data are expressed as mean ± SEM (n = 3). Values were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001. BrdU: 5-Bromo-2’-deoxyuridine; dpi: days post injury; GCL: ganglion cell layer; INL: inner nuclear layer; MO; morpholino; MGPC: Müller glia-derived progenitor cells; ns: not significant; ONL: outer nuclear layer; PBS: phosphate-buffered saline.