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SDS-PAGE of purified active preparations of ISPBPH chimeras. Lane 1, P6 ht-ISPBPH, 50 pmol; lane 2, LB400 ISPBPH, 80 pmol; lane 3, B-356 ISPBPH, 50 pmol; lane 4, Mr marker; lane 5, ht-αP6βLB400, 70 pmol; lane 6, ht-αP6βB-356, 170 pmol; lane 7, ht-αLB400βP6, 60 pmol; lane 8, ht-αB-356βP6, 50 pmol. P6 ht-ISPBPH, ht-αP6βLB400, and ht-αP6βB-356 were produced from P. putida KT2440; the other chimeras were produced from E. coli M15. Sizes are indicated on the left in kilodaltons.
