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. 2022 Jun 2;18(2):434–438. doi: 10.4103/1673-5374.346468

Figure 6.

Figure 6

Effect of TREML2 knockdown on microglial polarization markers.

(A) Levels of the microglial M1-type polarization marker iNOS as well as the microglial M2-type polarization markers CD206 and ARG1 in primary microglia after TREML2 knockdown, as detected by western blot assay. (B) Quantitative analysis of iNOS protein levels. Data were normalized to β-actin. (C) Quantitative analysis of CD206 protein levels. Data were normalized to β-actin. (D) Quantitative analysis of ARG1 protein levels. Data were normalized to β-actin. All figures show data representative of four independent experiments performed in duplicate. Data were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. Columns represent mean ± SD. *P < 0.05, vs. mock group; #P < 0.05, vs. LPS + sh-NC group. ARG1: Arginase 1; iNOS: inducible nitric oxide synthase; LPS: lipopolysaccharide (100 ng/mL); LPS+sh-NC: LPS+control for lentiviral knockdown particles; LPS+sh-TREML2: LPS+TREML2 shRNA lentiviral knockdown particles.