REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse Anti-Human IgG1-Fc PE	Southern Biotech	CAT# 9054-09; RRID: AB_2796628
Mouse Anti-Human IgG2-Fc PE	Southern Biotech	CAT# 9060-09; RRID: AB_2796635
Mouse Anti-Human IgG3-Fc PE	Southern Biotech	CAT# 9210-09; RRID: AB_2796701
Mouse Anti-Human IgM-Fc PE	Southern Biotech	CAT# 9020-09; RRID: AB_2796577
Mouse Anti-Human IgA1-Fc PE	Southern Biotech	CAT# 9130-09; RRID: AB_2796656
Mouse Anti-Human IgA2-Fc PE	Southern Biotech	CAT# 9140-09; RRID: AB_2796664
Pacific Blue(TM) anti-human CD66b antibody	Biolegend	CAT# 305112; RRID: AB_2563294
Chemicals, peptides, and recombinant proteins
Shigella recombinant proteins: IpaB, IpaC, IpaD, VirG and Shigella LPS	N/A	Provided by WRAIR
Shigella IpaH		Provided by Vaxcyte
BSA conjugated Shigella flexneri 2a O-polysaccharide	N/A	Isolated and produced at the Ryan lab/MGH, conjugated to BSA at NIH
Zaire ebolavirus glycoprotein	R&D Systems	CAT#9016-EB
Human Fc receptors	Produced at the Duke Human Vaccine Institute, {Boesch, 2014 #15}	N/A
Streptavidin-R-Phycoerythrin	Prozyme	CAT# PJ31S
EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)	Thermo Fisher	CAT# 77,149
Sulfo-NHS-LC-LC biotin	Thermo Fisher	CAT# A35358
DMTMM (4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride)	Sigma Aldrich	74,104
Lyophilized guinea pig complement	Cedarlane	CL4051
Software and algorithms
IntelliCyt ForeCyt (v8.1)	Sartorius	https://intellicyt.com/products/software/
FlowJo (v10.7.1)	FlowJo, LLC	https://www.flowjo.com/solutions/flowjo
Prism 9.2.0 (283)	GraphPad	https://www.graphpad.com/scientific-software/prism/
Other
FluoSpheres™ NeutrAvidin™-Labeled Microspheres, 1.0 μm, yellow-green fluorescent (505/515), 1% solids	Invitrogen	CAT# F8776
FluoSpheres™ Carboxylate-Modified Microspheres, 1.0 μm, blue (fluorescent 350/440), 1% solids	Invitrogen	CAT# F8815
FluoSpheres™ NeutrAvidin™-Labeled Microspheres, 1.0 μm, crimson fluorescent (625/645),1% solids	Invitrogen
FluoSpheres™ NeutrAvidin™-Labeled Microspheres, 1.0 μm, red-orange fluorescent (565/580)1% solids	Invitrogen
MagPlex microspheres	Luminex corporation	CAT# MC12001-01