Figure 2.

cDC1s fail to complete ESCRT-III-mediated repair

(A) Splenic cDCs were purified and left untreated (DMSO; top panel) or treated for 15 min (center panel) or 30 min (bottom panel) with 0.3 mM LLOME before fixation and galectin-3 and IST1 intracellular staining. Images shown were acquired with a confocal microscope equipped with a 40× objective (scale bar, 5 μm).

(B) Numbers and frequencies (mean) of GAL3/IST1 foci are the results of 2 independent experiments (Mann-Whitney t test, exact p-values are indicated). Each dot represents one cell. The numbers of cells analyzed are indicated in Table S4.