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. 2022 Aug 16;40(7):111205. doi: 10.1016/j.celrep.2022.111205

Figure 3.

Figure 3

ESCRT-III is recruited to damaged intracellular compartments and maintains their integrity

(A) Experiment timeline for PhagoFACS represented in (B).

(B) Recruitment of CHMP4b-mCherry on phagosomes was evaluated by flow cytometry. Plots and results representative of at least 2 independent experiments are shown in the left and right panels (two-way ANOVA, mean ± SEM, exact p values are indicated).

(C and D) CHMP4b-silenced, CHMP2a-silenced, or control cells were left untreated or incubated for 15 or 30 min with 0.5 mM LLOME before fixation and galectin-3 and IST1 staining. Images representative of 2 independent experiments are shown in (C) (scale bar, 5 μm). Ratio of area covered by IST1 foci on total cell area (left panel, mean, Student’s t test) and numbers of galectin-3 (right panel, Mann-Whitney t test) foci per cell are quantified in (D). Each dot represents one cell and exact p values are indicated. The numbers of cells analyzed are indicated in Table S4.