Figure 6.

RIPK3 kinase inhibition restricts antigen export to the cytosol in ESCRT-III-deficient DCs

(A–C) ESCRT-III-silenced and control MutuDCs were lysed at different times after transduction, and induction of necroptotic (A), apoptotic (B), and pyroptotic (C) mediators was assessed by immunoblotting. Data are representative of 2 independent experiments.

(D) Representative flow cytometry plots after 3 h of incubation with β-lactamase in the presence or absence of 10 μM GSK′872 (gated on live cells).

(E) Quantification of 2 experiments (two-way ANOVA, mean ± SEM, exact p values are indicated). The zero time point corresponds to incubation for 5 h, on ice, with β-lactamase.