Table 2.
Complete blood count results of 7 puppies infected with Hepatozoon canis at day 62 post-partum performed with ADVIA 2120i [Siemens Healthineers] in the Laboklin laboratory (Bad Kissingen, Germany) with age-related reference intervals according to Rortveit et al. (2015)
| Parameter | Reference interval | Puppy I | Puppy II | Puppy III | Puppy IV | Puppy V | Puppy VI | Puppy VII |
|---|---|---|---|---|---|---|---|---|
| RBC | 4.0–5.5 × 1012/l | 4.55 | 4.71 | 4.58 | 4.54 | 4.39 | 4.47 | 5.0 |
| HGB | 86–177 g/l | 98 | 109 | 105 | 103 | 100 | 106 | 111 |
| HCT | 0.27–0.37 l/l | 0.32 | 0.36 | 0.34 | 0.32 | 0.31 | 0.32 | 0.36 |
| MCV | 63.0–74.0 fl | 70.0 | 77.1 | 74.0 | 70.0 | 72.0 | 71.0 | 71.6 |
| MCH | Pga | 22.0 | 23.1 | 23.0 | 23.0 | 23.0 | 24.0 | 22.2 |
| MCHC | 29.0–34.0 g/dl | 31.0 | 30.0 | 31.0 | 33.0 | 32.0 | 33.0 | 31.0 |
| RET | /nla | 177.5 | 203.5 | 188.2 | 205.2 | 180.9 | 138.6 | 187.0 |
| CHr | pga | 23.0 | 24.4 | 23.6 | 23.5 | 22.8 | 26.3 | 24.0 |
| WBC | 8.8–22.4 × 109/l | 13.6 | 20.5 | 17.3 | 15.6 | 18.9 | 17.3 | 17.5 |
| Segb | 4.1–12.2 × 109/l | 6.8 | 9.8 | 6.7 | 7.2 | 7.9 | 8.1 | 11.9 |
| Lymphb | 2.7–11.3 × 109/l | 4.8 | 8.6 | 8.0 | 7.0 | 9.1 | 6.9 | 3.9 |
| Monob | 0.5–1.6 × 109/l | 1.4 | 1.0 | 1.7 | 0.9 | 1.5 | 0.9 | 0.9 |
| Eob | 0.1–1.8 × 109/l | 0.7 | 1.0 | 0.7 | 0.5 | 0.4 | 1.4 | 0.9 |
| Basob | × 109/l | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| Bandsb | < 0.5 × 109/l | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| PLT | 193–653 × 109/l | 241 | 399 | 367 | 429 | 280 | 393 | 307 |
| Hypochrb | Neg | Neg | Neg | Neg | Neg | Neg | Neg | Neg |
| Anisob | Neg | Neg | Neg | Neg | Pos | Neg | Neg | Neg |
| Hepatozoon gamontsc (day 62) (%) | 0 | 4 | 1 | 2 | 1 | 1.5 | 1 | 7 |
| Hepatozoon gamontsc (day 125) | 0% | 1% | 1% | – | – | 0% | 1% | 0% |
| Hepatozoon spp. PCR (day 62) | Negative | Positive (ct 32.8) | Positive (ct 34.8) | Positive (ct 31.8) | Positive (ct 32.6) | Positive (ct 31.1) | Positive (ct 31.5) | Positive (ct 31.8) |
| Hepatozoon spp. PCR (day 125) | Negative | Positive (ct 34.3) | Negative | – | – | Positive (ct 36.1) | Positive (ct 33.1) | Positive (ct 33.0) |
Bold values demonstrate parameters out of the diagnostic thresholds
RBC red blood cells; HGB haemoglobin; HCT haematocrit; RET reticulocytes; CHr reticulocyte haemoglobin content; WBC white blood cells; Seg segmented neutrophilic granulocytes; Lymph lymphocytes; Mono monocytes; Eo eosinophilic granulocytes; Baso basophilic granulocytes; Bands banded neutrophilic granulocytes; PLT platelets; Hypochrom hypochromasia; Aniso anisocytosis
aNo reference values provided by Rortveit et al. (2015)
bManual differential count (LABOKLIN GmbH & Co. KG.)
cManual count out of buffy-coat smear (LABOKLIN GmbH & Co. KG.)