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. 2022 Jul 15;17(8):2024–2030. doi: 10.1021/acschembio.2c00333

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© 2022 The Authors. Published by American Chemical Society

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(top) Diagram of the TOL2 plasmids containing N41-GFP, N41^R307Q-GFP, and GFP DNA under control of ubi promoter. (bottom) In situ hybridization for GFP mRNA in Tg(ubi:DDcAMP) (A), Tg(ubi:GFP) (D) and Tg(ubi:DDcAMP^R307Q) (G) embryos at 24 hpf. Confocal acquisition of GFP signal in Tg(ubi:DDcAMP) (B, C), Tg(ubi:GFP) (E, F), and Tg(ubi:DDcAMP^R307Q) (H, I) embryos at 24 hpf. Horizontal myoseptum is indicated by white arrows in panel B.