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SDS-PAGE analysis of S. aureus Cap5P expression and purification. Cell lysates were made from E. coli BL21(DE3) carrying pKBK7. Cap5P was overexpressed in the presence of 1 mM IPTG, and the soluble protein was purified over a nickel column. Lane 1, cell lysate from uninduced cells; lane 2, cell lysate from IPTG-induced cells; lane 3, column effluent reflecting unbound proteins; lane 4, purified S. aureus Cap5P. Molecular mass markers in kilodaltons are indicated on the left.
