TABLE 3.
Bacterial gene products with established or putative UDP-GlcNAc 2-epimerase activity
| Organism | Gene product | Size (aa)d | % Identity/similarity (aa stretch/gaps)a | Polysaccharide | Polysaccharide constituentsc | Demonstration of function (reference) |
|---|---|---|---|---|---|---|
| Staphylococcus aureus | Cap5P | 381 | 100/100 (381/0) | CP5 | ManNAcA, d-FucNAc, l-FucNAc | Complementation (9), in vitro enzymatic activity (this study) |
| Staphylococcus aureus | Cap8P | 381 | 99.2/99.2 (381/0) | CP8 | ManNAcA, d-FucNAc, l-FucNAc | NDe |
| Staphylococcus aureus | MnaA | 375 | 60.8/71.2 (375/0) | Teichoic acid linker? | ManNAc, GlcNAc | Complementation (this study) |
| Bacillus subtilis | YvyH/OrfXf | 380 | 59.0/70.0 (373/0) | Teichoic acid linker? | ManNAc, GlcNAc | ND |
| Streptococcus pneumoniae | Cps19fKb | 362 | 54.4/66.5 (357/1) | Type 19f capsule | ManNAc, glucose, l-rhamnose | Complementation (8) |
| Escherichia coli | RffE/NfrC | 376 | 51.9/60.8 (369/1) | ECA | ManNAcA, Fuc4NAc, GlcNAc | In vitro enzymatic activity (21) |
| Pseudomonas solanacearum | EpsC | 396 | 49.5/62.3 (374/1) | EPS I | GalNAc, GalNAcA, N-acetyl-2,4,6-trideoxygalactose | ND |
| Neisseria meningitidis | SacA | 372 | 48.7/61.8 (367/1) | Serogroup A capsule | ManNAc-1-P | ND |
| Salmonella enterica | WecB/RfbC | 378 | 47.9/59.9 (369/2) | LPS O:54 antigen | ManNAc | Complementation (18) |
| Pseudomonas aeruginosa | WbpI | 362 | 30.7/43.2 (328/4) | Serotype O5, band B LPS | 2,3-diNAcManA Man(2NAc3N)A FucNAc | ND |
| Bordatella pertussis | WlbD/BplD | 362 | 30.7/41.2 (365/6) | Band A LPS | 2,3-diNAcManA FucNAcMe GlcNAc | ND |
| Staphylococcus aureus | Cap5(8)G | 374 | 30.2/42.1 (367/9) | CP5(8) | ManNAcA, d-FucNAc, l-FucNAc | ND |
Sequences were aligned and compared with S. aureus Cap5P with the BestFit program (Genetics Computer Group).
Cps19fK is nearly identical to Cps19aK, Cps19bK, and Cps19cK.
2,3-diNAcManA, 2,3-dideoxy-2,3-di-N-acetyl-d-mannosaminuronic acid (2,3-diacetamido-d-mannuronic acid); Fuc4NAc, 4-acetamido-4,6-dideoxy-d-galactose; FucNAc, 2-acetamido-2,6-dideoxy-d-galactose (N-acetyl fucosamine); FucNAcMe, N-acetyl-N-methylfucosamine; GalNAc, N-acetyl-d-galactosamine; GalNAcA, N-acetyl-d-galactosaminuronic acid; Man(2NAc3N)A, 2-acetamido-3-acetamidino-2,3-dideoxy-d-mannuronic acid.
aa, amino acid.
ND, not determined (function based on sequence homology and putative biosynthetic pathway).
Alternative names for one gene product are separated by the slash (names given by different researchers or names that have been changed).