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. 2022 Aug 3;11(8):2880–2888. doi: 10.1021/acssynbio.2c00297

Figure 4.

Figure 4

Five weeks after being mixed into a composite potting soil, 10 ppb alachlor was detectable using a highly simplified assay with minimal sample preparation. The assay utilized a Y2H stain harboring mScarlet-I as an additional reporter and plasmid-encoded copies of GAL4-BD:BdPYL36A and GAL4-AD:ΔNBdPP2C44. Small pots with Arabidopsis seedlings containing an equal mass of composite soil were drenched in ALANEX (480 g/L alachlor) diluted in tap water to appropriate levels. Pots were allowed to dry up and were watered occasionally for 5 weeks. Sample preparation is described in the experimental section. Each data point is an average of 4 replicates, which are the same yeast culture mixed with the same soil sample and sectioned to four different wells for growth.