TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype or phenotype | Reference or construction |
|---|---|---|
| E. coli K-12 strains | ||
| BD1302 | ΔoxyR; autoaggregating | 13 |
| CC118λpir | λpir integrated on the chromosome | 19 |
| HEHA3 | BD1302 Δfim::kan | This study |
| HEHA10 | BD1302 flu::tet | This study |
| MS7 | PC31 Δfim::kan | 37 |
| PC31 | fim+ | 23 |
| Plasmids | ||
| pACYC184 | Camr Tetr | 10 |
| pBR322 | Ampr Tetr | 7 |
| pGP704 | Ampr; R6K-based origin (pir) | 19 |
| pHHA130 | oxyR+ | A 2,040-bp PCR fragment (primers 5 and 6) containing the oxyR gene from PC31 inserted into the HindIII site of pACYC184 |
| pHHA145 | StyI site deleted | pBR322 cut with StyI, made blunt with Klenow polymerase, and religated |
| pHHA146 | flu+ | A 3,550-bp PCR fragment containing the flu gene from PC31 inserted into the EcoRI/BamHI site of pBR322 |
| pHHA154 | flu+ | A 3,550-bp PCR fragment containing the flu gene from PC31 inserted into the EcoRI/BamHI site of pHHA145 |
| pHHA159 | flu::tet | A 1,724-bp BsaAI/SspI fragment containing the tet gene inserted into the (blunted) StyI site of pHHA154 |
| pHHA165 | flu::tet | A 5,450-bp EcoRI fragment containing the flu::tet construct inserted into the EcoRI site of pGP704 |
| pLBJ311 | Δfim::kan | 37 |
| pLIH14 | Has all fim genes except fimA | 25 |
| pMAS32 | lacUV5::fimACDFGH | Part of the fim gene cluster (fimA to fimH) inserted behind the isopropyl-β-d-thiogalactopyranoside-inducible lacUV5 promoter |
| pPAP5 | Contains the pap gene cluster | 27 |
| pPKL4 | Contains the fim gene cluster | 23 |
| pPKL5 | Contains the fimBEAICD genes | 21 |
| pPKL9 | fimB+ | 20 |
| pPKL143 | Contains the foc gene cluster | 22 |