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PCARE mutants are expressed and stable. Wild-type and mutant PCARE constructs were transfected in HEK293T cells. Western blot analysis revealed stable expression of all PCARE constructs. Wild-type PCARE protein has a molecular weight of 140 kDa, the molecular weight of mutant PCARE proteins is approximately the same, except for the mutant carrying the helix deletion which is smaller. Tubulin was loaded for control of the total amount of protein.
