TABLE 1.
E. coli strains used
| Strain | Genotype or description | Source or reference |
|---|---|---|
| AT980 | dapD2 relA1 spoT1 thi-1 Hfr(defective) | E. coli Genetic Stock Center (Yale University, New Haven, Conn.), isolate 4545 |
| AG100K | AG100 ΔmarORAB::Kanr | 23 |
| HSL5 | AT980 ΔmarCORAB::Kanr | P1(AG100K) × AT980 |
| HSL6 | AT980 ΔacrAB::Tn903 Kanr | P1(JZM120) × AT980 |
| TP982 | AT980 oppB::mini-Tn10Cm ampG::Kan | 29 |
| HSL2 | AT980 oppB::mini-Tn10Cm | P1(TP982) × AT980 |
| TP985 | AT980 mppA::mini-Tn10Cm | 29 |
| HSL3 | TP985 ΔacrAB::Tn903 Kanr | P1(JZM120) × TP985 |
| HSL4 | TP985 ΔmarCORAB::Kanr | P1(AG100K) × TP985 |
| AG100 | argE3 thi-3 rpsL xyl mtl supE44 Δ(gal-uvrB) | 10 |
| AG102 | AG100 marR1 | 10 |
| JZM120 | argE3 hisG4 leuB6 Δ(gpt-proA)62 thr-1 thi-1 ara-14 galK2 lacY1 mtl-1 xyl-1 kdgK51 tsx-33 recB21 recC22 sbcB15 supE44 rpsL31 rac ΔacrAB::Tn903 Kanr | 21 |
| JF568 | aroA357 ilv-227 metB65 his-53 purE41 cyc-1 xyl-14 lacY29 rpsL77 tsxN63 | 7 |
| JF701 | JF568 ompC264 | 7 |
| JF703 | JF568 ompF254 aroA+ | 7 |