Table 2.
The 20 novel microsatellite loci developed in the study along with their related PCR information.
| BAC Accession | Clone Name | SSR Locus | Chr | Primer Sequence (5’–3’) | Repeat Motif | Allele Size (s) (bp) | Tm (°C) |
|---|---|---|---|---|---|---|---|
| AC235792 | C03HBa0029M12 | ju003 | 3 | F-ATGGTGTGTCAGTCCTTTCATC | 8 GA | 254 | 50.4 |
| R-AAAGGTTAAGGGTCCTGCTAGC | 52.9 | ||||||
| AC235795 | C03HBa0036B17 | ju004 | 3 | F-TCGATGTCATTACTCACGTTCC | 5 CA | 238, 242 | 51.7 |
| R-GATACCAAAACGCAGCAAGTTG | 54.1 | ||||||
| AC235804 | C03HBa0224P23 | ju006 | 10 * | F-CATTTCATGAAAGGGGAATTCTAG | 10 TG | 201, 277 | 53.1 |
| R-ACATTTCGTGTTAGCTGGGTTC | 52.6 | ||||||
| AC238438 | C03HBa0031F10 | ju 007 | 3 | F-GAGTTTGATAAAGCAAAAGGC | 6 AG | 163, 182 | 48.2 |
| R-AACAGAACCCGAGTTTGGAC | 50.5 | ||||||
| AC238439 | C03HBa0031P17 | ju008 | 3 | F-CAATTATTAGACAGCCAACCAAG | 5 AAT | 264 | 50.5 |
| R-GGCATTTATTTGGTCAGAAAGC | 52.5 | ||||||
| AC238450 | C03HBa0114P24 | ju010 | 3 | F-TACCCTTTCGTTTACCCAAATTG | 11 AT | 282 | 54.1 |
| R-AATTGACCGATTTTCCCTTCTC | 53.1 | ||||||
| AC238451 | C03HBa0121O11 | ju011 | 3 | F-GTGAAATGATGTTTCCTCTGACAAG | 5 AAC | 246, 253 | 53.7 |
| R-CTTTCGACATCCTTTTGACTCG | 53.2 | ||||||
| AC238457 | C03HBa0166B15 | ju014 | 3 | F-CGGCAATGTAAGAGTTGAGCTC | 6 GA | 243 | 53.4 |
| R-ATCATCCCAAGCGTCAAAATAG | 52.7 | ||||||
| AC238459 | C03HBa0176B05 | ju015 | 3 | F-ACTCTTCATCCGTTGTACAATTC | 6 TTC | 264, 276 | 49.5 |
| R-TTCACTCGGATGATTGTAATCG | 51.9 | ||||||
| AC238462 | C03HBa0203H10 | ju017 | 3 | F-GATTTTATTGGGTGTCTGTTGTC | 5 TGT | 248 | 49.8 |
| R-AGGGAGAAAAGATGAACAGTATC | 48.1 | ||||||
| AC238468b | C12HBa0270F24 | ju022 | 12 ** | F-ATGGATTTACTGTAACAGTGTGAAC | 6 TTC | 293 | 49 |
| R-GTCCAAATTAATAACAGATCCATAG | 48.4 | ||||||
| AC238468c | C12HBa0270F24 | ju023 | 12 ** | F-AATTATTCGTAAGTTTCCGTCTGTC | 25 AT | 308, 320 | 52.2 |
| R-CCTTTATGAATGACCAAAAGCTAC | 51.3 | ||||||
| AC238560 | C03HBa0030A11 | ju026 | 3 | F-AATCAATATCATCGCTTCACTG | 19 TA | 246, 292 | 48.9 |
| R-ATGTTGTGGTATTATTGACTTATGAG | 48.7 | ||||||
| AC238561 | C03HBa0031M05 | ju027 | 3 | F-ATGCTTAAGGTCTCCAAACACC | 5 CAA | 250 | 51.8 |
| R-CTCTCTACTTTTGGGATTACGC | 49.7 | ||||||
| EU124730 | C03HBa0001E24 | ju029 | 3 | F-TGCTGTACATACTGCATAAATGG | 7 TG | 350 | 50.1 |
| R-AACCTGCTGAATTAACTTGTAGTG | 49.5 | ||||||
| EU124737 | C03HBa0054O21 | ju035 | 3 | F-GTTATATAGAAAGACAAGGTAGAAGGTC | 25 AT | 288, 293 | 49.7 |
| R-GGTAGACTTTTTATGTGTTGTTGC | 49.7 | ||||||
| EU124739 | C03HBa0233O20 | ju037 | 3 | F-AAAATTGTTGGTCAACATGGTG | 7 TAT | 241, 246 | 51.6 |
| R-TTATCTCCTTTCCCTTTCATTC | 49 | ||||||
| EU124741 | C04HBa0318C22 | ju039 | 4 | F-GATGGTGTCATAGATCTAGCCTTAG | 6 TTAA | 355, 421 | 50.4 |
| R-TGGGGAATTATGTAGTGTTGAG | 48.7 | ||||||
| EU124742 | C03HBa0323D22 | ju040 | 3 | F-GCGATCCTGTTTGAGAAGAAGG | 5 CA | 340, 345 | 54.6 |
| R-ATGAACAAATGCTTAAGAGGGG | 52 | ||||||
| EU124743 | C03HBa0007J09 | ju041 | 3 | F-TTCCAAAAACACTTACGAAAGTTAG | 26 AT | 292, 316, 330 | 51.4 |
| R-CATGTAAGTCAAAAGAATGGAGG | 50.2 |
* The original clones was assigned to chromosome number 3 (clone number C03HBa0224P23). ** The original clones was assigned to chromosome number 3 (clone number C03HBa0270F24).